We tested 130 sera collected from patients there were three periods:before transplantation, 6 month and 12 months after transplantation. DynaChip and LabScreen Luminex methods were used to identify anti-HLA antibody against HLA-A,B,C,DR,DQ and DP. We were able to define specificity of antibodies on the low and high resolution level. Software DynaChip and Fusion were used to perform immunogenetic analyses of our research.
In a group of patients 57 % people expressed anti-HLA antibodies. We identified anti-HLA antibodies against HLA class I and HLA class II. We were able to define specificity of antibodies on low and high resolution level. In a group of 43 % of patients we did not identify anti-HLA antibodies. We devided a patient into two groups mismatched in HLA class I antigen and mismatched in HLA class II antigen. We analysed people produced antibody against one or many antigens. As a second step of our research we analyse donor recipient mismatches on the level of allele. Donor recipient mismatches were connected with HLA allele locus C (6,6 % ), HLA allele locus B (13,3 % ), HLA allele locus A (3,3 % ), HLA allele locus DQB1?(10 % ). When we look at the PRA positive patient 20 % of them express presence of antibodies against many specificities, 16,7 % against no more than 3 specificity, 20 % against single antigen.
As a continuation of our research we are going to use the Immport Database. We would like to try compare specificity of anti-HLA antibodies and molecular structure of mismatched alleles.
Siekiera: Blood Center: Other: HLA Lab Director. Dobrowolska: Blood Center: Employee. Markiewicz: Medical University of Silesia: Other: Clinician.