SMART Digestℿcompared with pellet digestion for analysis of human immunoglobulin G1 in rat serum by liquid chromatography tandem mass spectrometry

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• 作者：Christian Lanshoeft^a ; ^b ; Olivier Heudi^a ; ^{olivier.heudi@novartis.com" class="auth_mail" title="E-mail the corresponding author} ; Sarah Cianf&eacute ; rani^b
• 关键词：SMART Digest&trade ; Pellet digestion ; Generic peptide ; Human immunoglobulin G1 ; Liquid chromatography tandem mass spectrometry
• 刊名：Analytical Biochemistry
• 出版年：2016
• 出版时间：15 May 2016
• 年：2016
• 卷：501
• 期：Complete
• 页码：23-25
• 全文大小：304 K

文摘

The newly developed SMART Digest™ kit was applied for the sample preparation of human immunoglobulin G1 (hIgG1) in rat serum prior to qualitative and quantitative analyses by liquid chromatography tandem mass spectrometry (LC–MS/MS). The sequence coverages obtained for the light and heavy chains of hIgG1A were 50 and 76%, respectively. The calibration curve was linear from 1.00 to 1000 μg/ml for three of four generic peptides. Overall, the SMART Digest™ kit resulted in similar quantitative data (linearity, sensitivity, accuracy, and precision) compared with the pellet digestion protocol. However, the SMART Digest™ required only 2 h of sample preparation with fewer reagents.