Heterogeneous nuclear ribonucleoproteins F and H/H′ show differential expression in normal and selected cancer tissues
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- 作者：Honoré ; Bent ; Baandrup ; Ulrik ; Vorum ; Henrik
- 关键词：qRRM family of hnRNPs ; hnRNP F ; hnRNP H/H&prime ; RNA processing ; Gene expression ; Differential expression ; Differential regulation ; Immunohistochemistry
- 刊名：Experimental Cell Research
- 出版年：2004
- 出版时间：March 10, 2004
- 年：2004
- 卷：294
- 期：1
- 页码：199-209
- 全文大小：1.52 M
文摘
The heterogeneous nuclear ribonucleoproteins (hnRNPs) F and H/H′, containing the quasi-RNA recognition motif (qRRM) domains, are implicated in several steps of pre-mRNA processing and in cellular differentiation. We have compared a set of tissues and found striking differences in their levels of expression as well as in the nuclear versus the cytoplasmic distribution. Generally, hnRNP F is broadly expressed in many tissues with extremely strong expression in the prostate gland while hnRNP H/H′ shows a more restricted degree of expression with low expression in some tissues, for example, liver, exocrine acini of the pancreas, thyroid gland and heart. At the cellular level, hnRNP F is, with few exceptions, predominantly expressed in the cytoplasm while hnRNP H/H′ is more abundant in the nuclei. A quite pronounced heterogeneous expression pattern is seen in the proximal tubules of the kidney where hnRNP F is present at moderate cytoplasmic levels while hnRNP H/H′ is undetectable, whereas both proteins are more evenly expressed in distal tubules and collecting ducts. Generally, tumor tissues reveal a broad expression of hnRNP F in the nuclei as well as in the cytoplasm while hnRNP H/H′ is expressed at higher levels in the nuclei than in the cytoplasm. Up-regulation of hnRNP H/H′ is found in a few tissues that normally express low cytoplasmic levels of hnRNP H/H′, for example, adenocarcinoma of the pancreas, hepatocellular carcinoma and gastric carcinoma. hnRNP F is down-regulated in hepatocellular carcinoma and up-regulated in gastric carcinoma. The present study indicates the important potential role of this subset of hnRNPs on the gene expression in many tissues.