Measurement of O6-alkylguanine-DNA alkyltransferase activity in tumour cells using stable isotope dilution HPLC-ESI⿿MS/MS
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- 作者：Guohui Sun sunguohui@emails.bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Lijiao Zhao ; zhaolijiao@bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Tengjiao Fan fantengjiao2014@emails.bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Ting Ren renting@bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Rugang Zhong lifesci@bjut.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：AGT ; O6-alkylguanine-DNA alkyltransferase ; HPLC-ESI⿿MS/MS ; high-performance liquid chromatography electrospray ionization tandem mass spectrometry ; O6-BG ; O6-benzylguanine ; Cys145 ; Cysteine 145 ; O6-MG ; O6-methylguanine ; [3H]MNU ; N-[3H]-methyl-N-nitrosourea ; UV ; ultraviolet ; NMR ; nuclear magnetic resonance ; MEM-EBSS ; Eagle⿿s minimal essential medium with Earle⿿s balanced salt solution ; FBS ; foetal bovine serum ; DTT ; dithiothreitol ; PMSF ; phenylmethanesulfonyl fluoride ; CID ; collision induced diss
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：1033-1034
- 期：Complete
- 页码：138-146
- 全文大小：1918 K
文摘
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An LC⿿MS/MS-based method for measuring AGT activity was established.
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AGT activity can be accurately quantified by the nonradioactive method.
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The novel method was applied for determining AGT activity in tumor cells.
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The method is promising in evaluating drug resistance to chemotherapies.