The Nucleolar PICT-1/GLTSCR2 Protein Forms Homo-Oligomers
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- 作者：Tatyana Borodianskiy-Shteinberg ; Inna Kalt ; Sarit Kipper ; Nofar Nachum ; Shiri Katz ; Maor H. Pauker ; Mira Barda-Saad ; Doron Gerber ; Ronit Sarid ; saridr@mail.biu.ac.il" class="auth_mail
- 关键词：PICT-1 ; protein interacting with carboxyl terminus 1 ; ES ; embryonic stem ; FRET ; fluorescence resonance energy transfer ; DBD ; DNA binding domain ; AD ; activation domain ; MBP ; maltose-binding protein ; ECFP ; enhanced cyan fluorescent protein ; EYFP ; enhanced yellow fluorescent protein ; HEK-293T ; human epithelial kidney 293T ; PBS ; phosphate-buffered saline ; BSA ; bovine serum albumin
- 刊名：Journal of Molecular Biology
- 出版年：12 June 2014
- 年：2014
- 卷：426
- 期：12
- 页码：2363-2378
- 全文大小：1192 K
文摘
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PICT-1 is a nucleolar protein that regulates cell fate and nucleolar stress response.
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PICT-1 may function as a tumor suppressor or as an oncogene.
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Self-association of PICT-1 is shown in yeast and in mammalian cells.
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Microfluidic assays identified the carboxy-terminus as mediating self-interaction.
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Another layer of complexity to the different functions of PICT-1 is added.