Biochemical characterization of plasmepsin V from Plasmodium vivax Thailand isolates: Substrate specificity and enzyme inhibition
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- 作者：Khomkrit Sappakhawa ; Ratchaneekorn Takasilaa ; Pichamon Sittikula ; Pakorn Wattana-Amornb ; c ; Wanchai Assavalapsakuld ; Nonlawat Boonyalaia ; b ; nonlawat.b@ku.ac.th" class="auth_mail" title="E-mail the corresponding author ; nonlawat@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：DABCYL ; 4-((4-(dimethylamino)phenyl)azo)benzoic acid ; DMSO ; dimethyl sulfoxide ; EDANS ; 5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid ; FRET ; fluorescence resonance energy transfer ; HAP ; histo aspartic protease ; HIV-1 ; human immunodeficiency virus-1 ; HRPII ; histidine-rich protein II ; PfEMP2 ; Plasmodium falciparum erythrocyte membrane protein 2 ; PfPMV ; Plasmodium falciparum plasmepsin V ; PMSF ; phenyl-methylsulphonyl fluoride ; PTEX ; Plasmodium falciparum translocon of exported proteins ; PvPMV
- 刊名：Molecular & Biochemical Parasitology
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：204
- 期：2
- 页码：51-63
- 全文大小：3039 K
文摘
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PvPMV-Thai contains a four-amino acid insertion (SVSE).
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The SVSE insertion does not interfere with the catalytic activity.
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The negatively charged amino acid at the P2 position of the PEXEL motif is unfavorable.
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Menisporopsin A partially inhibits the PvPMV-Thai activity at high concentration.