A label-free silver wire based impedimetric immunosensor for detection of aflatoxin M1 in milk

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• 作者：Gautam Bacher^a ; ^b ; ¹  ; [Author Vitae] ; Souvik Pal^a ; ¹  ; [Author Vitae] ; Lizy Kanungo^a  ; [Author Vitae] ; Sunil Bhand^a ; ^{sunil17_bhand@yahoo.com} ; ^{sunilbhand@bits-goa.ac.in}  ; [Author Vitae]
• 关键词：Immunosensor ; Aflatoxin M1 ; Silver wire electrode ; Electrochemical impedance spectroscopy ; Label-free detection ; Milk
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2012
• 出版时间：20 June, 2012
• 年：2012
• 卷：168
• 期：Complete
• 页码：223-230
• 全文大小：805 K

文摘

A highly sensitive and selective label-free impedimetric immunosensor based on silver (Ag) wire electrode for the detection aflatoxin M1 (AFM1) in milk is presented. The sensor was constructed by functionalizing Ag wire coupled with selective monoclonal antibodies of AFM1 through self assembled monolayers. The antigen-antibody interaction was quantified by measuring impedance in the frequency range (1-100 Hz) at 10 mV applied ac potential. A linear working range (6.25-100 pg mL^?) for AFM1 was obtained with 20 min analysis time. The sensor sensitivity was found to be about 2.1 % impedance change per decade with limit of quantitation 6.25 pg mL^? and limit of detection 1 pg mL^?. Good recoveries were obtained with SD = 1.13 and R² = 0.982 in spiked milk samples. The proposed method is useful for sensitive analysis of AFM1 in milk.