A capillary zone electrophoresis method for adenine nucleotides analysis in Saccharomyces cerevisiae
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- 作者：Pan Zhua ; Song Wanga ; Jian Wanga ; Li Zhoua ; Ping Shia ; b ; ship@ecust.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Saccharomyces cerevisiae ; Capillary zone electrophoresis ; Adenosine triphosphate ; Adenosine diphosphate ; Adenosine monophosphate ; Cyclic adenosine monophosphate
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 January 2016
- 年：2016
- 卷：1008
- 期：Complete
- 页码：156-163
- 全文大小：1411 K
文摘
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A novel capillary zone electrophoresis method was established to analyze adenine nucleotides from Saccharomyces cerevisiae extract.
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Validation analyses suggest that the method is reliable and accurate with high recovery and precision in the range of linearity.
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It is the first time that ATP, ADP, AMP, and cAMP were detected simultaneously in yeast.
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The current method may contribute to further metabolomics and system biology investigations of yeast.