Fast separation and analysis of reduced monoclonal antibodies with capillary zone electrophoresis coupled to mass spectrometry
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- 作者：Yimeng Zhaoa ; Liangliang Suna ; Michael D. Kniermanb ; Norman J. Dovichia ; ndovichi@nd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Reduced monoclonal antibody ; Capillary zone electrophoresis ; Mass spectrometry ; Sample preparation
- 刊名：Talanta
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：148
- 期：Complete
- 页码：529-533
- 全文大小：1289 K
文摘
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We developed a sample preparation protocol for reduced antibody CE–MS analysis.
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Heavy chain and light chain were baseline resolved for a single antibody within 10 min.
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Heavy chains and light chains were partially resolved for a mixture of two antibodies.