Chemotypes significance of lichenized fungi by structural characterization of heteropolysaccharides from the genera Parmotrema and Rimelia

详细信息    查看全文

• 作者：Carbonero ; Elaine Rosechrer ; Mellinger ; Caroline Grassi ; Eliasaro ; Sionara ; Gorin ; Philip Albert James ; et. al.
• 关键词：Lichenized fungi ; Parmeliaceae ; Galactoglucomannan ; Chemical structure ; ¹³C NMR
• 刊名：FEMS Microbiology Letters
• 出版年：2005
• 出版时间：May 15, 2005
• 年：2005
• 卷：246
• 期：2
• 页码：273-278
• 全文大小：161 K

文摘

Galactoglucomannans were isolated from the lichenized fungi of the genus Parmotrema (Parmotrema austrosinense, Parmotrema delicatulum, Parmotrema mantiqueirense, Parmotrema schindlerii, and Parmotrema tinctorum and that of Rimelia (Rimelia cetrata and Rimelia reticulata) via successive hot alkaline extraction and precipitation with Fehling solution. The structure of each polysaccharide was investigated using ¹³C NMR and HSQC-DEPT spectroscopy, methylation analysis, and HPSEC-MALLS. The galactoglucomannans had a (1 → 6)-linked main chain of α-Manp units, substituted preferentially at O-2 and O-4 by α-Galp and β-Galp nonreducing end-units, respectively. The C-1 region of the ¹³C NMR spectra of these heteropolysaccharides is typical of the lichen species, and is an additional tool in lichenized fungi classification.