A tumor progression model derived from a new T24 HRAS transformed NIH/3T3 cell line.
T1-A, T2-A, T3-HA, T4PA lines derived from GhrasT-NIH/3T3, T1-A, T2-A, T3-HA tumors.
PCR detected the human HRAS oncogene in T2-A and T4-PA metastatic tumors.
These five new cell lines showed a progressive increase in tumorigenic potential.
New method obtained doubling times using daily photomicrographs of gridded plates.