A CMV DNA vaccine primes for memory immune responses to live-attenuated CMV (Towne strain)

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• 作者：Mark A. Jacobson ; Stuart P. Adler ; Elizabeth Sinclair ; Douglas Black ; Anna Smith ; Alice Chu ; Ron B. Moss ; Mary K. Wloch
• 关键词：Cytomegalovirus ; Vaccine ; T lymphocytes ; Cytokines
• 刊名：Vaccine
• 出版年：2009
• 出版时间：4 March 2009
• 年：2009
• 卷：27
• 期：10
• 页码：1540-1548
• 全文大小：470 K

文摘

CMV-seronegative subjects vaccinated intramuscularly or intradermally with a DNA vaccine encoding pp65, IE1, and gB were administered live-attenuated CMV (Towne) to characterize immune priming by the DNA vaccine. CMV-specific memory T-cells (detected by standard ELISPOT assay in only 20 % of subjects) were detected by IFN-γ cultured ELISPOT assay in 60 % of subjects primed intramuscularly and correlated with immune responses after Towne. The median time to first pp65 T-cell and gB antibody response after Towne was 14 days for DNA-primed subjects vs. 28 days for controls administered Towne only (p = 0.02 and 0.03, respectively). Furthermore, there was a trend toward more DNA-vaccinated subjects than controls developing a gB-specific IFN-γ T-cell response after Towne administration (47 % vs. 0 % , p = 0.06).