Sodium fluoride induces hypercalcemia resulting from the upregulation of both osteoblastic and osteoclastic activities in goldfish, Carassius auratus

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• 作者：Masayuki Sato^a ; Taizo Hanmoto^a ; Koji Yachiguchi^a ; Yoshiaki Tabuchi^b ; Takashi Kondo^c ; Masato Endo^d ; Yoichiro Kitani^a ; Toshio Sekiguchi^a ; Makoto Urata^a ; ^e ; Tran Ngoc Hai^f ; Ajai K. Srivastav^g ; Hiroyuki Mishima^h ; Atsuhiko Hattoriⁱ ; Nobuo Suzuki^a ; ^{nobuos@staff.kanazawa-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ALP ; alkaline phosphatase ; CTSK ; cathepsin K ; EF1α ; elongation factor 1α ; MMP-9 ; matrix metalloproteinase-9 ; NaF ; sodium fluoride ; NFATc1 ; nuclear factor of activated T-cells and cytoplasmic 1 ; TRAF6 ; TNF receptor-associated factor 6 ; TRAP ; tartrate-resistant acid phosphatase ; OPG ; osteoprotegerin ; RANK ; receptor activator of the nuclear factor-κB ; RANKL ; receptor activator of the nuclear factor-κB ligand
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：189
• 期：Complete
• 页码：54-60
• 全文大小：555 K

文摘

The influence of sodium fluoride (NaF) on calcium metabolism was examined in goldfish (fresh water teleost). At 2 days after administration of NaF (500 ng/g body weight; 5 μg/g body weight) (around 10^− 5 to 10^− 4 M in goldfish), we indicated that plasma calcium levels upregulated in both doses of NaF-treated goldfish. To examine the mechanism of hypercalcemia by NaF treatments, therefore, direct effects of NaF on osteoblasts and osteoclasts in goldfish were investigated by an original assay system using teleost scale which has osteoblasts, osteoclasts and bone matrix. Alkaline phosphatase activity in the scales increased with the treatment of NaF (10^− 6 and 10^− 5 M) during 6 h of incubation. Also, tartrate-resistant acid phosphatase activity increased after exposure to NaF (10^− 5 M) at the 6 h of incubation. To investigate the osteoclastic activation, the mRNA expression of osteoclastogenesis related factors were examined. The receptor activator of the nuclear factor-κB ligand (RANKL) which is known as a factor for osteoclastogenesis, increased in the NaF-treated scales after 6 h of incubation. The ratio of RANKL/osteoprotegerin (osteoclastogenesis inhibitory factor) significantly increased after 6 h of incubation. Resulting from the increase of RANKL mRNA level, the expression of transcription-regulating factors was significantly increased. Furthermore, the expression of functional genes, cathepsin K and matrix metalloproteinase-9 mRNA, was significantly increased. In our knowledge, this is the first report concerning the effects of NaF on osteoblasts and osteoclasts in teleosts. We concluded that NaF influences calcium metabolism via osteoclastic activation in goldfish.