Simulation of elution profiles in liquid chromatography—I: Gradient elution conditions, and with mismatched injection and mobile phase solvents
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- 作者：Lena N. Jeonga ; Ray Sajulgab ; Steven G. Fortea ; Dwight R. Stollb ; dstoll@gustavus.edu" class="auth_mail" title="E-mail the corresponding author ; Sarah C. Rutana ; srutan@vcu.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sample/eluent solvent mismatch ; Sample volume overload ; Linear solvent strength theory ; Simulation
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：29 July 2016
- 年：2016
- 卷：1457
- 期：Complete
- 页码：41-49
- 全文大小：835 K
文摘
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An extension of the LC simulation method of Czok and Guiochon is described.
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Results for isocratic and gradient elution, overload and solvent mismatch are given.
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Excellent agreement is found between simulations, experimental results and theory.
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The simulations help to characterize effects of LC parameters on performance.
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The simulations are useful when departing significantly from ‘standard’ conditions.