Transcriptional regulation of IL-17A and other inflammatory markers during the development of soybean meal-induced enteropathy in the distal intestine of Atlantic salmon (Salmo salar L.)

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• 作者：Inderjit S. Marjara ; Elvis M. Chikwati ; Elin C. Valen ; ?shild Krogdahl ; Anne Marie Bakke
• 关键词：SBM ; Cytokines ; Inflammation ; TH17 cells ; IL-17A
• 刊名：Cytokine
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：60
• 期：1
• 页码：186-196
• 全文大小：1545 K

文摘

Progression of soybean meal (SBM)-induced enteropathy in Atlantic salmon (m>Salmo salarm> L.) distal intestine (DI) was studied to investigate pathophysiological mechanisms and immune responses. Seawater-adapted salmon were fed an extracted SBM-containing diet (200 g kg^?1) from day 1-21 and compared with fish fed a fishmeal-based diet (day 0). Histological evaluation of the DI revealed signs of inflammation from day 5, which progressively increased in severity and affected more fish with increasing SBM exposure time. The expression profiles of 16 genes were analyzed by quantitative PCR. The pro-inflammatory cytokines interleukin 17A (IL-17A), IL-1¦Â, interferon ¦Á (IFN¦Á) and IFN¦Ã, as well as IL-17A receptor, T-cell receptor ¦Ã (TCR¦Ã), cluster of differentiation 4¦Á (CD4¦Á), CD8¦Â, transforming growth factor ¦Â (TGF¦Â), trypsin, protease-activated receptor 2 (PAR2) and myeloid differentiation primary response gene 88 (MyD88) were significantly up-regulated during early and/or late inflammation stages, whereas interferon-¦Ã-inducible lysosomal thiol reductase (GILT) was downregulated. Up-regulation of TCR¦Ã from day seven suggests proliferation of intraepithelial ¦Ã¦Ä T cells. IL-17A, up-regulated by 218-fold during early inflammation, indicates involvement of T helper 17 cells in the pathogenesis of the SBM-induced inflammatory response.