Real-time and non-invasive monitoring of embryonic stem cell survival during the development of embryoid bodies with smart nanosensor

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• 作者：Jiayin Fu^a ; ¹ ; Christian Wiraja^a ; ¹ ; Ruiqi Chong^a ; Chenjie Xu^a ; ^b ; ^{CJXu@ntu.edu.sg} ; Dong-An Wang^a ; ^{DAWang@ntu.edu.sg}
• 关键词：Embryonic stem cell ; Embryoid body ; Viability ; Nanosensor ; Real-time and non-invasive monitoring
• 刊名：Acta Biomaterialia
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：49
• 期：Complete
• 页码：358-367
• 全文大小：3032 K
• 卷排序：49

文摘

Embryonic stem cells (ESCs)-derived embryoid body (EB) is a powerful model for the study of early embryonic development and the discovery of therapeutics for tissue regeneration. This article reports a smart nanosensor platform for labeling and tracking the survival and distribution of ESCs during the EB development in a real-time and non-invasive way. Compared with the cell tracker (i.e. DiO) and the green fluorescent protein (GFP), nanosensors provide the homogenous and highly-efficient ESC labeling. Following the internalization, intracellular nanosensors gradually release the non-fluorescent molecules that become fluorescent only in viable cells. This allows a continuous monitoring of ESC survival and distribution during the process of EB formation. Finally, we confirm that nanosensor labeling does not cause the significant influences to biological properties of the ESCs and EBs.Statement of SignificanceThe distribution pattern of viable embryonic stem cells (ESCs) within embryoid body (EB) is closely related with the maturation of EBs. Noninvasive and real-time monitoring of viable ESC distribution in EBs would allow researchers to optimize the culturing condition in time during the EB development and to select the suitable EBs for subsequent applications.