Proximate and ultimate sources of individual diet variation were investigated in a marine fish assemblage/ Proximate sources of individual diet variation suggested by Optimal Foraging Theory were supported/ Ultimate sources of individual diet variation related to the Niche Variation Hypothesis were only partly supported/ Partial resource partitioning was revealed as higher individual diet variation resulted in limited increase in niche overlap/ Combining various trophic niche metrics allows inferring competition effects on communities’ trophic niche organization