Studies of endothelial monolayer formation on irradiated poly-l-lactide acid with ions of different stopping power and velocity

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• 作者：Claudia R. Arbeitman^a ; ^b ; ^c ; Mariela F. del Grosso^a ; ^b ; Irene L. Ibañ ; ez^a ; Moni Behar^d ; Mariano Grasselli^c ; Gerardo Garcí ; a Bermú ; dez^b ;
• 关键词：PLLA ; Irradiated polymers ; Swift heavy ions ; Cell adhesion ; Cell proliferation
• 刊名：Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms
• 出版年：2015
• 出版时间：15 December 2015
• 年：2015
• 卷：365
• 期：part_PB
• 页码：587-591
• 全文大小：1343 K

文摘

In this work we study cell viability, proliferation and morphology of bovine aortic endothelial cells (BAEC) cultured on poly-l-lactide acid (PLLA) modified by heavy ion irradiation. In a previous study comparing ions beams with the same stopping power we observed an increase in cell density and a better cell morphology at higher ion velocities. In the present work we continued this study using heavy ions beam with different stopping power and ion velocities. To this end thin films of 50 μm thickness were irradiated with 2 MeV/u and 0.10 MeV/u ion beams provided the Tandar (Buenos Aires, Argentina) and Tandetron (Porto Alegre, Brazil) accelerators, respectively. The results suggest that a more dense and elongated cell shapes, similar to the BAEC cells on the internal surface of bovine aorta, was obtained for stopping power of 18.2–22.1 MeV cm² mg⁻¹ and ion velocity of 2 MeV/u. On the other hand, for low ion velocity 0.10 MeV/u the cells present a more globular shapes.