Generation and characterization of cells that can be conditionally depleted of mitochondrial SOD2

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• 作者：Shunya Takada ; Eri Inoue ; Keizo Tano ; Hanako Yoshii ; Takuya Abe ; Akari Yoshimura ; Motomu Akita ; Shusuke Tada ; Masami Watanabe ; Masayuki Seki ; Takemi Enomoto
• 关键词：DT40 ; Mn– ; SOD ; Cell cycle ; SOD2 ; ROS ; SOD1
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2009
• 出版时间：6 February 2009
• 年：2009
• 卷：379
• 期：2
• 页码：233-238
• 全文大小：486 K

文摘

Manganese-dependent superoxide dismutase (SOD2) serves as the primary defense against mitochondrial superoxide, and decreased SOD2 activity results in a range of pathologies. To investigate the events occurring soon after depletion of SOD2, we generated SOD2 gene knockout chicken DT40 cells complemented with a human SOD2 (hSOD2) cDNA, whose expression can be switched off by doxycycline (Dox). When SOD2 was depleted by the addition of Dox, the cells grew slightly slower and formed fewer colonies than cells expressing hSOD2. In addition, these cells showed a high sensitivity to paraquat, which produces superoxide, and died through apoptosis. In contrast to results obtained with mouse and Drosophila Sod2 mutants, we found no indication of an increase in DNA lesions due to depletion of SOD2.