Increased monocyte/neutrophil and pro-coagulant microparticle levels and overexpression of aortic endothelial caveolin-1β in dyslipidemic sand rat, Psammomys obesus

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• 作者：Mohamed El Fadel Ousmaal^a ; ^b ; ^{ousmaal_med@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; M. Carmen Martí ; nez^c ; ^{carmen.martinez@univ-angers.fr" class="auth_mail" title="E-mail the corresponding author} ; Ramaroson Andriantsitohaina^c ; ^{ramaroson.andriantsitohaina@univ-angers.fr" class="auth_mail" title="E-mail the corresponding author} ; Kahina Chabane^b ; ^{kahinachabane@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; Abderahim Gaceb^c ; ^{fond.bleu@hotmail.fr" class="auth_mail" title="E-mail the corresponding author} ; Saâ ; dia Mameri^d ; ^{saadiamameri@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Jean Giaimis^e ; ^{jean.giaimis@univ-montp1.fr" class="auth_mail" title="E-mail the corresponding author} ; Ahsene Baz^b ; ^{baznacim@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Dyslipidemia ; Microparticle ; CD11b ; Phosphatidylserine ; Caveolin-1 ; Psammomys obesus
• 刊名：Journal of Diabetes and its Complications
• 出版年：2016
• 出版时间：January-February 2016
• 年：2016
• 卷：30
• 期：1
• 页码：21-29
• 全文大小：1124 K

文摘

To compare the effects of a high-energy diet (HED) with those of a low-energy diet (LED) on biochemical parameters, microparticle (MP) subpopulations and endothelial caveolin-1 (cav-1) protein expression in Psammomys obesus (P. obesus).

Methods

After 12 weeks of feeding with either the HED or LED, fasting plasma glucose and lipid parameters were measured using an enzymatic colorimetric kit while serum insulin concentration was determined with radioimmunoassay kits. MP subpopulations and cav-1 protein expression were quantified using flow cytometry and western blot analysis, respectively.

Results

We observed that the HED caused a marked increase in lipid parameters, even in normoglycemic P. obesus. The total number of circulating MPs and the numbers of platelet-, leukocyte-, and erythrocyte-derived MPs were unaltered in the HED group. However, the HED induced increases in the numbers of monocytes/neutrophils and procoagulant MPs and a decrease in the endothelial MP levels. Cav-1β protein expression and reactive oxygen species production were increased in the vascular endothelium of HED-treated P. obesus.

Conclusion

From these findings, it is indicated that the HED exerts deleterious effects on the vascular system by increasing the monocyte/neutrophil and procoagulant MP levels, which may lead to cav-1β protein overexpression in dyslipidemic P. obesus.