Houttuynia cordata Thunb. polysaccharides ameliorates lipopolysaccharide-induced acute lung injury in mice

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• 作者：Yan-Yan Xu^a ; Yun-Yi Zhang^a ; ^{zhcheng@shmu.edu.cn" class="auth_mail" title="E-mail the corresponding author} ; Ying-Ye Ou^a ; Xiao-Xiao Lu^a ; Ling-Yu Pan^a ; Hong Li^a ; Yan Lu^b ; Dao-Feng Chen^b ; ^{dfchen@shmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：HCP ; Houttuynia cordata polysaccharides ; ALI ; acute lung injury ; LPS ; lipopolysaccharide ; DEX ; dexamethasone or Dexamethasone Sodium Phosphate Injection ; TNF-伪 ; tumor necrosis factor-伪 ; IL-6 ; interleukin-6 ; IL-1尾 ; interleukin-1尾 ; ELISA ; enzyme-linked immunosorbent assay ; NS ; normal saline ; NO ; nitric oxide ; HRP ; horseradish peroxides ; DAB ; 3 ; 3&prime ; -diaminobenzidine ; VBS2+ ; isotonic veronal-buffered saline ; EAs ; sensitized erythrocytes ; w/d ; wet/dry weight ratio ; PB ; polymyxin B ; BALF ; bronchoa
• 刊名：Journal of Ethnopharmacology
• 出版年：2015
• 出版时间：15 September 2015
• 年：2015
• 卷：173
• 期：Complete
• 页码：81-90
• 全文大小：12782 K

文摘

Houttuynia cordata (HC) has been used as a folk therapy to treat pulmonary infections. This study aimed to determine the role and mechanism of action of polysaccharides isolated from HC (HCP) in lipopolysaccharide (LPS)-induced ALI in the mice.

Materials and methods

LPS was delivered by the intratracheal route to Balb/c mice 2 h before HCP (40, 80 and 160 mg/kg) administration.

Results

The number of total cells, protein and tumor necrosis factor-伪 (TNF-伪) concentrations in bronchoalveolar lavage fluid, the wet/dry weight ratio (w/d) of lungs and pulmonary pathology of each mouse were analyzed, it was found that HCP significantly alleviated ALI induced by LPS. Moreover, in lungs of mice, it was found that the infiltration of inflammatory cells, the expression of Toll-like receptor 4 and complement deposition were significantly decreased by HCP treatment. In vitro assays showed that C5a, a complement activation product, induced significant macrophage migration and treatment with HCP prevented it. The in vitro results also proved that LPS increased nitric oxide and pro-inflammatory cytokines (TNF-伪, interleukin-6, and interleukin-1尾) production, and HCP antagonized these effects of LPS. It was also found that HCP alone augmented secretion of some pro-inflammatory cytokines.

Conclusion

These results indicate that HCP may alleviate LPS induced lung inflammatory injury, which may be associated with its inhibitory effect on the over activation of complement and macrophages. This suggests a potential role to treat ALI.