Dissecting the polysaccharide-rich grape cell wall matrix using recombinant pectinases during winemaking
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- 作者：Yu Gaoa ; ; Jonatan U. Fangelb ; William G.T. Willatsb ; Melané ; A. Viviera ; John P. Moorea ; moorejp@sun.ac.za" class="auth_mail" title="E-mail the corresponding author
- 关键词：AIR ; alcohol insoluble residue ; AGP ; arabinogalactan protein ; ARA ; endo-arabinase ; CoMPP ; comprehensive microarray polymer profiling ; CBM ; carbohydrate binding module ; CDTA ; cyclohexanediamine-tetra-acetic acid ; DE ; degree of esterification ; EPG ; endo-polygalacturonase ; FT-IR ; fourier transform-infrared spectroscopy ; GAL ; endo-galactanase ; GC ; gas chromatography ; HG ; homogalacturonan ; mAb ; monoclonal antibody ; PL ; pectin-lyase ; PME ; pectin-methyl-esterase ; RG ; rhamnogalacturonan
- 刊名：Carbohydrate Polymers
- 出版年：2016
- 出版时间：5 November 2016
- 年：2016
- 卷：152
- 期：Complete
- 页码：510-519
- 全文大小：3276 K
文摘
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Recombinant pectinases were used during the winemaking process.
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CoMPP datasets were analysed using multivariate methods.
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Pectin lyase showed strong de-pectination action on grapes.
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Other pectinase combinations unraveled but did not de-pectinate.
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A hypothetical grape berry cell wall model was proposed.