Innate immune gene expression in individual zebrafish after Listonella anguillarum inoculation

详细信息    查看全文

• 作者：Irune Rojo ; Ó ; skar Martí ; nez de Ilá ; rduya ; Andone Estonba ; Miguel Á ; ngel Pardo
• 关键词：Danio rerio ; Cytokines ; il1b ; cebpb ; Immunity ; Real-time RT-PCR ; Variability
• 刊名：Fish & Shellfish Immunology
• 出版年：2007
• 出版时间：December 2007
• 年：2007
• 卷：23
• 期：6
• 页码：1285-1293
• 全文大小：263 K

文摘

Zebrafish were intraperitoneally injected with 10⁶ CFU (LD₅₀) Listonella anguillarum. Three inoculated and control fish were collected at 1, 2, 4, 6 and 22 h post infection (hpi) and the expression of genes related to the immune response (il1b, cebpb, tfa, mpx, tnfa, nitr9, tlr22, hsc70, cp, mrlp1, c3b and lyz) in each fish was monitored by means of real-time RT-PCR. A similar experiment was performed considering an intermediate time point at 15 hpi. Different relative levels of expression were found among genes. Also, wide interindividual variation in gene expression for most genes was detected among fish, inoculated or not.

A steady increase of expression starting from the initial stages of the interaction was found for interleukin-1β. An initial increase in levels of gene expression was found for the genes coding for the CCAAT/Enhancer Binding Protein subunit β and the Novel Immune-Type Receptor 9, although their levels decreased later on and were indistinguishable from the controls at 22 hpi. Finally, some genes (Transferrin, Myeloid-specific Peroxidase and Tumour Necrosis Factor α) were upregulated at 22 hpi.

Taken together, our results show an induction in gene expression of genes involved in the inflammatory and immune response upon L. anguillarum infection but also reveal the existence of a wide variation in the levels of expression of the studied genes in the zebrafish population.