文摘
The hippocampus receives cholinergic afferents and expresses neuronal nicotinic acetylcholine receptors. In particular, the α7 and 946;2 nicotinic subunits are highly expressed in the hippocampus. There has been controversy about the location, distribution and roles of neuronal nicotinic acetylcholine receptors [Role L. W. and Berg D. K. (1996) Neuron 16, 1077-1085; Wonnacott S. (1997) Trends Neurosci. 20, 92-98]. Using immunocytochemistry and patch-clamp techniques, we examined the density and distribution of nicotinic receptors on rat hippocampal neurons in primary tissue culture. The density and distribution of α7 subunits change with days in culture. Before 10 days in culture, α7 expression, monitored immunocytochemically, is low and nicotinic currents are small or absent. In older cultures, about two-thirds of the neurons express nicotinic currents, and α7 appears in small patches on the soma and out along the neuronal processes. These patches of α7 subunits on the surface of the neuronal processes often co-localize with the presynaptic marker, synaptotagmin. The other most common nicotinic subunit, 946;2, stays confined mainly to the soma and proximal processes, and 946;2 is distributed more uniformly and is not specifically localized at presynaptic areas. The two subunits, α7 and 946;2, have different expression patterns on the surface of the cultured hippocampal neurons.