Optimal extracellular production of recombinant Bacillus circulans β-galactosidase in Escherichia coli BL21(DE3)

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• 作者：Xuguo Duan^a ; ^b ; ^c ; Shubing Hu^a ; ^b ; Xuhui Qi^a ; ^b ; Zhengbiao Gu^a ; ^d ; ^e ; Jing Wu^a ; ^b ; ^{jingwu@jiangnan.edu.cn}
• 关键词：Escherichia coli ; Extracellular production ; β-galactosidase ; Optimization
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：17-24
• 全文大小：1101 K
• 卷排序：53

文摘

β-Galactosidase production conditions (induction time, temperature, concentration of lactose, etc) were optimized in a 3-L fermentor. The highest extracellular enzyme activity was 18.5-fold that of the original extracellular enzyme activity in 3-L fermenter. Peak extracellular β-galactosidase activity reached 220.0 U mL−1.