Testing the dependence of stabilizing effect of osmolytes on the fractional increase in the accessible surface area on thermal and chemical denaturations of proteins
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- 作者：Safikur Rahman1 ; Syed Ausaf Ali ; Asimul Islam ; Md. Imtaiyaz Hassan ; Faizan Ahmad ; fahmad@jmi.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：Osmolytes ; Methylamines ; Protein stabilization ; Accessible surface area ; Gibbs free energy
- 刊名：Archives of Biochemistry and Biophysics
- 出版年：2016
- 出版时间：1 February 2016
- 年：2016
- 卷：591
- 期：Complete
- 页码：7-17
- 全文大小：1738 K
文摘
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To assess the extent of stabilization by osmolytes, denaturation of barstar, cyt-c and Mb by GdmCl and heat were performed.
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The larger the change in accessible surface area on denaturation, the more is the stabilization of proteins by osmolytes.
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Except GB, there exists a good correlation between theoretical and experimental m-values for all GdmCl-denatured proteins.