Function of alternative splicing

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• 作者：Olga Kelemen¹ ; Paolo Convertini¹ ; Zhaiyi Zhang ; Yuan Wen ; Manli Shen ; Marina Falaleeva ; Stefan Stamm ; ^{stefan@stamms-lab.net}
• 关键词：CLIP ; cross link and immunoprecipitation ; CTD ; carboxy terminal domain ; Cyr61 ; cysteine rich 61 ; ESE ; exonic sequence enhancer ; HITS-CLIP ; high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation ; hnRNP ; heterogenous ribonuclear prot
• 刊名：Gene
• 出版年：2013
• 出版时间：1 February, 2013
• 年：2013
• 卷：514
• 期：1
• 页码：1-30
• 全文大小：1454 K

文摘

Almost all polymerase II transcripts undergo alternative pre-mRNA splicing. Here, we review the functions of alternative splicing events that have been experimentally determined. The overall function of alternative splicing is to increase the diversity of mRNAs expressed from the genome. Alternative splicing changes proteins encoded by mRNAs, which has profound functional effects. Experimental analysis of these protein isoforms showed that alternative splicing regulates binding between proteins, between proteins and nucleic acids as well as between proteins and membranes. Alternative splicing regulates the localization of proteins, their enzymatic properties and their interaction with ligands. In most cases, changes caused by individual splicing isoforms are small. However, cells typically coordinate numerous changes in ¡®splicing programs¡¯, which can have strong effects on cell proliferation, cell survival and properties of the nervous system. Due to its widespread usage and molecular versatility, alternative splicing emerges as a central element in gene regulation that interferes with almost every biological function analyzed.