A detailed approach to study the antibacterial mechanisms of nanostructure

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• 作者：Fang Fang^a ; ^b ; ^{fangfang1982131@yahoo.com.cn} ; Xuan Fang^a ; ^b ; Jinhua Li^a ; ^b ; Zhipeng Wei^a ; Xin Wang^a ; Xiaohua Wang^a
• 关键词：ZnO ; Nanomaterial ; Antibacterial ; CLSM
• 刊名：Applied Surface Science
• 出版年：2012
• 出版时间：1 March, 2012
• 年：2012
• 卷：258
• 期：10
• 页码：4397-4401
• 全文大小：547 K

文摘

To overcome the limitations of the conventional inhibition method for mechanism study, here we developed a combined CLSM technique as an alternative approach to evaluate and explain the antibacterial process of nanostructure at single cell level. Three different nanostructures (dumbbell-shaped ZnO, rod-shaped ZnO and ZnO/Ag composites) were examined here as a model system. Their antibacterial processes against Escherichia coli (E. coli) were quantitatively studied in detail. According to the obtained time-dependent mean fluorescence intensity (MFI) decay dynamics, an interesting two-step cellular disruption process was observed and discussed for the first time. The combined CLSM technique described here is superior to standard growth method for they can provide a more detailed and reliable description of cell-nanostructure interactions.