Adsorptive anodic stripping differential pulse voltammetric determination of CellCept at Fe3O4 nanoparticles decorated multi-walled carbon nanotubes modified glassy carbon electrode
详细信息 查看全文
- 作者：Mohammad Bagher Gholivand ; mbgholivand2013@gmail.com ; mbgholivand@yahoo.com ; Mohammad Solgi
- 关键词：Cellcept ; Stripping voltammetry ; MWCNTS ; Fe3O4 magnetic nanoparticles
- 刊名：Analytical Biochemistry
- 出版年：2017
- 出版时间：1 March 2017
- 年：2017
- 卷：520
- 期：Complete
- 页码：1-8
- 全文大小：1532 K
- 卷排序：520
文摘
A simple and sensitive method based on adsorptive anodic stripping differential pulse voltammetry (AASDPV) for the determination of cellcept, using a magnetic Fe3O4 nanoparticles and functionalized (carboxylated) multi-walled carbon nanotubes modified glassy carbon electrode (f-MWCNs/Fe3O4/GCE) was developed. In phosphate buffer solution (pH = 5), the voltammogram of cellcept exhibited tow anodic peaks and the well-defined peak at about 0.611 V vs SCE was used for its monitoring. The modified electrode was characterized by different methods such as electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The experimental parameters, such as pH, deposition potential and time, as well as scan rate were optimized. Under the optimized conditions, Ip (μA) was proportional to the cellcept concentration in the range of 0.05–200 μM (R2 = 0.9989) with a detection limit of 9.0 nM and limit of quantification of 30.2 nM. The recovery was >98%. The practical analytical utilities of the modified electrode were demonstrated by the determination of cellcept in human urine and blood serum samples. Modified electrode showed an adequate sensitivity and stability for evaluated samples.