A peptide mimetic of an anti-CD4 monoclonal antibody by rational design
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- 作者：Casset ; Florence ; Roux ; Florence ; Mouchet ; Patrick ; Bes ; Cedric ; Chardes ; Thierry ; Granier ; Claude ; et. al.
- 关键词：Antibody modeling ; Antibody mimetic ; Paratope mimetic ; Rational design ; Anti-CD4 monoclonal antibody ; ST40
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2003
- 出版时间：July 18, 2003
- 年：2003
- 卷：307
- 期：1
- 页码：198-205
- 全文大小：311 K
文摘
The development of rational methods to design ‘continuous’ sequence mimetics of discontinuous regions of protein sequence has, to now, been only marginally successful. This has been largely due to the difficulty of constraining the recognition elements of a mimetic structure to the relative conformational and spatial orientations present in the parent molecule. Using peptide mapping to determine ‘active’ antigen recognition residues, molecular modeling, and a molecular dynamics trajectory analysis, we have developed a peptide mimic of an anti-CD4 antibody, containing antigen contact residues from multiple CDRs. The design described is a 27-residue peptide formed by juxtaposition of residues from 5 CDR regions. It displays an affinity for the antigen (CD4) of 0.9nM, compared to 2nM for the parent antibody ST40. Nevertheless, the mimetic shows low biological activity in an anti-retroviral assay.