Short communication: Labeling Listeria with anaerobic fluorescent protein for food safety studies

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• 作者：José ; M. Landete ; ^{landete.josem@inia.es} ; Á ; ngela Peiroté ; n ; Margarita Medina ; Juan L. Arqué ; s
• 关键词：Listeria ; anaerobic fluorescent protein ; food safety studies
• 刊名：Journal of Dairy Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：100
• 期：1
• 页码：113-117
• 全文大小：239 K
• 卷排序：100

文摘

Many food safety-related studies require the tracking of inoculated food-borne pathogens to monitor their fate in food complex environments. In the current study, we demonstrate the potential of plasmids containing the fluorescence protein gene evoglow-Pp1 (Evocatal, Dusseldorf, Germany) as a real-time reporter system for Listeria strains. This anaerobic fluorescent protein provides an easily detectable phenotype of microorganisms for food safety studies. This work is the first to report a reliable method to identify fluorescently labeled Listeria strains in food ecosystems.