Development of a subgenomic clone system for Kyasanur Forest disease virus

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• 作者：Bradley W.M. Cook^a ; ^b ; ^d ; ¹ ; Aidan M. Nikiforuk^a ; ^b ; ^d ; ¹ ; Todd A. Cutts^a ; ^b ; Darwyn Kobasa^c ; ^e ; Deborah A. Court^d ; Steven S. Theriault^a ; ^b ; ^d ; ^{steven.theriault@phac-aspc.gc.ca" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Kyasanur Forest disease virus ; Tick-borne flavivirus ; Flavivirus ; Subgenomic clone system ; Molecular genetics ; Antiviral research
• 刊名：Ticks and Tick-borne Diseases
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：7
• 期：5
• 页码：1047-1051
• 全文大小：692 K

文摘

Emerging tropical viruses pose an increasing threat to public health because social, economic and environmental factors such as global trade and deforestation allow for their migration into previously unexposed populations and ecological niches. Among such viruses, Kyasanur Forest disease virus (KFDV) deserves particular recognition because it causes hemorrhagic fever. This work describes the completion of an antiviral testing platform (subgenomic system) for KFDV that could be used to quickly and safely screen compounds capable of inhibiting KFDV replication without the requirement for high containment, as the structural genes have been replaced with a luciferase reporter gene precluding the generation of infectious particles. The coordination of KFDV kinetics with the replication characteristics of the subgenomic system has provided additional insight into the timing of flavivirus replication events, as the genetically engineered KFDV genome began replication as early as 2 h post cellular entry. Possession of such antiviral testing platforms by public health agencies should accelerate the testing of antiviral drugs against emerging or recently emerged viruses mitigating the effects of their disease and transmission.