Optimized and validated spectrophotometric methods for the determination of amiodarone hydrochloride in commercial dosage forms using N-bromosuccinimide and bromothymol blue

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• 作者：Nafisur Rahman^a ; ^{cht17nr_amu@yahoo.com} ; SK Manirul Haque^b ; Syed Najmul Hejaz Azmi^c ; Habibur Rahman^a
• 关键词：Spectrophotometry ; Amiodarone hydrochloride ; N-bromosuccinimide ; Bromothymol blue ; Commercial dosage forms
• 刊名：Journal of Saudi Chemical Society
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：21
• 期：1
• 页码：25-34
• 全文大小：1014 K
• 卷排序：21

文摘

Two optimized and validated spectrophotometric methods have been developed for the determination of amiodarone hydrochloride in commercial dosage forms. Method A is based on the reaction of tertiary amino group of the drug with N-bromosuccinimide in methanol–acetone medium resulting in the formation of a yellow colored product, which absorbed maximally at 353 nm. Method B involves the formation of a colored chloroform extractable ion-pair complex of the drug with bromothymol blue at pH 2.32 absorbing maximally at 400 nm. Beer’s law is obeyed in the concentration ranges 50–600 and 2–55 μg mL−1 with molar absorptivity of 1.58 × 102 and 1.50 × 104 L mol−1 cm−1 for methods A and B, respectively. The regression analysis yields the calibration equations: A = 4.012 × 10−4 + 1.16 × 10−3 C and A = 9.612 × 10−4 + 2.198 × 10−2 C for methods A and B, respectively. The application of the proposed methods to commercial dosage forms is presented.