cAMP-dependent protein kinase regulates post-translational processing and expression of complex I subunits in mammalian cells

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• 作者：Sergio Papa ; Salvatore Scacco ; Domenico De Rasmo ; Anna Signorile ; Francesco Papa ; Damiano Panelli ; Annarita Nicastro ; Raffaella Scaringi ; Arcangela Santeramo ; Emilio Roca ; Raffaella Trentadue ; Maria L
• 关键词：Complex I ; cAMP cascade ; PKA ; Mitochondrial import ; All trans retinoic acid ; Cell growth
• 刊名：Biochimica et Biophysica Acta (BBA)/Bioenergetics
• 出版年：2010
• 出版时间：June-July 2010
• 年：2010
• 卷：1797
• 期：6-7
• 页码：649-658
• 全文大小：1137 K

文摘

Work is presented on the role of cAMP-dependent protein phosphorylation in post-translational processing and biosynthesis of complex I subunits in mammalian cell cultures. PKA-mediated phosphorylation of the NDUFS4 subunit of complex I promotes in cell cultures in vivo import/maturation in mitochondria of the precursor of this protein. The import promotion appears to be associated with the observed cAMP-dependent stimulation of the catalytic activity of complex I. These effects of PKA are counteracted by activation of protein phosphatase(s). PKA and the transcription factor CREB play a critical role in the biosynthesis of complex I subunits. CREB phosphorylation, by PKA and/or CaMKs, activates at nuclear and mitochondrial level a transcriptional regulatory cascade which promotes the concerted expression of nuclear and mitochondrial encoded subunits of complex I and other respiratory chain proteins.