Silicalites and Mesoporous Silica Nanoparticles for photodynamic therapy

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• 作者：Ouahiba Hocine ; Magali Gary-Bobo ; David Brevet ; Marie Maynadier ; Simon Fontanel ; Laurence Raehm ; Sé ; bastien Richeter ; Bernard Loock ; Pierre Couleaud ; Cé ; line Frochot ; Clarence Charnay ; Gaë
• 关键词：Nanoparticles ; Mesoporous ; Organized porosity ; Silica ; PDT ; Targeting ; Mannose
• 刊名：International Journal of Pharmaceutics
• 出版年：2010
• 出版时间：15 December 2010
• 年：2010
• 卷：402
• 期：1-2
• 页码：221-230
• 全文大小：603 K

文摘

The synthesis of silicalites and Mesoporous Silica Nanoparticles (MSN), which covalently incorporate original water-soluble photosensitizers for PDT applications is described. PDT was performed on MDA-MB-231 breast cancer cells. All the nanoparticles showed significant cell death after irradiation, which was not correlated with ¹O₂ quantum yield of the nanoparticles. Other parameters are involved and in particular the surface and shape of the nanoparticles which influence the pathway of endocytosis. Functionalization with mannose was necessary to obtain the best results with PDT due to an active endocytosis of mannose-functionalized nanoparticles. The quantity of mannose on the surface should be carefully adjusted as a too high amount of mannose impairs the phototoxicity of the nanoparticles. Fluorescein was also encapsulated in MCM-41 type MSN in order to localize the nanoparticles in the organelles of the cells by confocal microscopy. The MSN were localized in lysosomes after active endocytosis by mannose receptors.