Bronchodilators modulate inflammation in chronic obstructive pulmonary disease subjects

详细信息    查看全文

• 作者：Pierachille Santus ; Carola Buccellati ; Stefano Centanni ; Francesca Fumagalli ; Paolo Busatto ; Francesco Blasi ; Angelo Sala
• 关键词：Neutrophils ; Leukotriene B4 ; Superoxide anion production ; Chronic obstructive pulmonary disease ; Bronchodilators
• 刊名：Pharmacological Research
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：66
• 期：4
• 页码：343-348
• 全文大小：446 K

文摘

Chronic obstructive pulmonary disease (COPD) is characterized by neutrophilic airway inflammation and oxidative stress. Leukotriene B₄ (LTB₄), a potent proinflammatory mediator, is synthesized by 5-lipoxygenase (5-LO), which is activated by the presence of lipid hydroperoxides resulting from oxidative stress on biological membranes.

We proposed to evaluate the effect of a four week treatment with two different bronchodilators of common practice in COPD treatment, on the production of reactive oxygen species (ROS), in particular superoxide anions, and of LTB₄ by peripheral blood neutrophils obtained from COPD subjects.

24 subjects among the COPD outpatients were enrolled, and randomized to receive either formoterol (12 ¦Ìg bid) or tiotropium (18 ¦Ìg od). Peripheral blood neutrophils were obtained at the start and at the end of the treatment, and production of superoxide anions and of LTB₄ were evaluated as previously published.

The results obtained showed a decrease in the unstimulated production of superoxide by isolated neutrophils in both groups, but tiotropium only was effective in modulating the production of LTB₄, while formoterol caused an increased production of superoxide in response to fMLP, when compared to values obtained before treatment.

In conclusion, tiotropium showed a better antiinflammatory activity profile when compared to formoterol in a clinical setting, reducing superoxide and LTB₄ production by peripheral neutrophils obtained from COPD subjects.