Insights in understanding aggregate formation and dissociation in cation exchange chromatography for a structurally unstable Fc-fusion protein
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- 作者：Zhiqiang Chena ; Chao Huanga ; Naresh Chennamsettyb ; Xuankuo Xua ; xuankuo.xu@bms.com" class="auth_mail" title="E-mail the corresponding author ; Zheng Jian Lia
- 关键词：Cation-exchange chromatography ; Fc-fusion protein ; Aggregate formation ; Aggregate dissociation ; Structurally unstable proteins
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：19 August 2016
- 年：2016
- 卷：1460
- 期：Complete
- 页码：110-122
- 全文大小：2195 K
文摘
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Aggregation phenomena in CEX step were studied for a structurally unstable Fc-fusion protein.
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Conformational changes in bound protein affect eluate aggregate level and aggregation reversibility.
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Conditions that improve the structural integrity of bound protein help reduce aggregate formation.
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CEX resin hydrophobicity contributes significantly to protein-resin interactions for partially unfolded protein.
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An agarose resin immobilized with multi-functional ligands was prepared to verify the proposed hypothesis.