The intra-group homogeneity of a marker is vital to estimate wound age accurately.
The intra-group homogeneity of SFRP5, FZD4 and Fosl1mRNA was detected and compared.
The intra-group homogeneity was calculated using CV of CVs (coefficient of variation).
The intra-group homogeneity of FZD4 mRNA was better than SFRP5 and Fosl1 mRNAs.
mRNA encoding membranous proteins are recommend to estimate wound age accurately.