In vivo and in vitro analyses of α-galactosylceramide uptake by conventional dendritic cell subsets using its fluorescence-labeled derivative
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- 作者：Maki UshidaaAuthor Vitae ; Tomonori Iyodaa ; 1Author Vitae ; Mitsuhiro Kanamoria ; 2Author Vitae ; Hiroshi WataraibAuthor Vitae ; Kazuhiko Takaharaa ; ktakahar@zoo.zool.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding authorAuthor Vitae ; Kayo InabaaAuthor Vitae
- 关键词：αGC ; α-galactosylceramide ; cDCs ; conventional dendritic cells ; Cy5-αGC ; Cy5-conjugated αGC ; iNKT ; cells invariant natural killer T cells
- 刊名：Immunology Letters
- 出版年：2015
- 出版时间：December 2015
- 年：2015
- 卷：168
- 期：2
- 页码：300-305
- 全文大小：1435 K
文摘
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We saw the fate of fluorescent α-galactosylceramide (Cy5-αGC) in vivo and in vitro.
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CD8+ dendritic cells (DCs) captured more αGC than CD8− DCs in vivo and in vitro.
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Cy5-αGC was more stable in CD8+ DCs than in CD8− DCs.
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Langerin+CD8+ DCs in the splenic marginal zone incorporated Cy5-αGC.
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Splenic CD8+ DCs expressed more CXCL16, which might anchor iNKT cells, than CD8− DCs.