Selective quantitation of the incorporation of the immunomodulator α-galactosylceramide in liposomes using LC-MS/MS
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- 作者：Kan Kanekoa ; kanka749@student.otago.ac.nz" class="auth_mail" title="E-mail the corresponding author ; Arlene McDowella ; arlene.mcdowell@otago.ac.nz" class="auth_mail" title="E-mail the corresponding author ; Yasuyuki Ishiib ; ishiiyas@rcai.riken.jp" class="auth_mail" title="E-mail the corresponding author ; Sarah Hooka ; sarah.hook@otago.ac.nz" class="auth_mail" title="E-mail the corresponding author
- 关键词：α-GalCer ; α-Galactosylceramide ; Chol ; cholesterol ; CV% ; coefficient of variation ; DPPC ; dipalmitoylphosphatidylcholine ; FDA ; Food and Drug Administration ; iNKT ; invariant natural killer T ; MRM ; multiple reaction monitoring ; QC ; quality control ; SD ; standard deviation
- 刊名：International Journal of Mass Spectrometry
- 出版年：2015
- 出版时间：3 December 2015
- 年：2015
- 卷：392
- 期：Complete
- 页码：96-101
- 全文大小：617 K
文摘
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A validated method for selectively quantitating α-GalCer in DPPC/Chol liposomes was developed.
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We have demonstrated the application of the method for determining incorporation efficiency with varying α-GalCer concentrations.
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The method has utility for monitoring the stability of α-GalCer incorporation in liposome formulations.