Multiple reaction monitoring and multiple reaction monitoring cubed based assays for the quantitation of apolipoprotein F
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- 作者：Abhinav Kumar ; abhinav.kumar@bioch.ox.ac.uk" class="auth_mail" title="E-mail the corresponding author ; Bevin Gangadharan ; Nicole Zitzmann
- 关键词：LCMS ; Liquid chromatography mass spectrometry ; HPLC ; High pressure liquid chromatography ; SRM ; Selective reaction monitoring ; MRM ; Multiple reaction monitoring ; MRM3 ; multiple reaction monitoring cubed ; APO-F ; Apolipoprotein F ; BSA ; Bovine serum albumin ; 2D gel ; Two dimensional gel ; LTIP ; Lipid transfer inhibitor protein ; LDL ; Low density lipoproteins ; HDL ; High density lipoproteins ; CETP ; Cholesteryl ester transfer protein ; FASP ; Filter-aided sample preparation ; AQUA ; Absolute QUAntitation ; FCS
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 October 2016
- 年：2016
- 卷：1033-1034
- 期：Complete
- 页码：278-286
- 全文大小：1750 K
文摘
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Liver fibrosis biomarker assay by LC⿿MS.
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Absolute quantification of APO-F in human plasma by MRM.
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Peptide selection and library creation using Skyline for MRM.
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Reduction of interference by MRM3.
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Presence of glycopeptide by MRM and Enhanced product ion scanning.