Human YKL39 (chitinase 3-like protein 2), an osteoarthritis-associated gene, enhances proliferation and type II collagen expression in ATDC5 cells

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• 作者：Kazumasa Miyatake^a ; Kunikazu Tsuji^b ; ^{ktsuji.gcoe@tmd.ac.jp} ; Mika Yamaga^a ; Jun Yamada^a ; Yu Matsukura^a ; Kahaer Abula^a ; Ichiro Sekiya^c ; Takeshi Muneta^a ; ^b
• 关键词：hYKL-39 ; Chi-lectin ; Osteoarthritis ; Rheumatoid arthritis
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：1 February, 2013
• 年：2013
• 卷：431
• 期：1
• 页码：52-57
• 全文大小：396 K

文摘

Human YKL39 (chitinase 3-like protein 2/CHI3L2) is a secreted 39 kDa protein produced by articular chondrocytes and synoviocytes. Recent studies showed that hYKL-39 expression is increased in osteoarthritic articular chondrocytes suggesting the involvement of hYKL-39 in the progression of osteoarthritis (OA). However little is known regarding the molecular function of hYKL-39 in joint homeostasis. Sequence analyses indicated that hYKL-39 has significant identity with the human chitotorisidase family molecules, although it is considered that hYKL-39 has no enzymatic activity since it lacks putative chitinase catalytic motif. In this study, to examine the molecular function of hYKL-39 in chondrocytes, we overexpressed hYKL-39 in ATDC5 cells. Here we report that hYKL-39 enhances colony forming activity, cell proliferation, and type II collagen expression in these cells. These data suggest that hYKL-39 is a novel growth and differentiation factor involved in cartilage homeostasis.