Increased production of L-serine in Escherichia coli through Adaptive Laboratory Evolution

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• 作者：Hemanshu Mundhada^a ; Jose M. Seoane^a ; Konstantin Schneider^a ; Anna Koza^a ; Hanne B. Christensen^a ; Tobias Klein^a ; Patrick V. Phaneuf^b ; Markus Herrgard^a ; Adam M. Feist^a ; ^b ; ^{afeist@ucsd.edu} ; Alex T. Nielsen^a ; ^{atn@biosustain.dtu.dk}
• 关键词：ALE ; Adaptive Laboratory Evolution ; MAGE ; Mutiplex Genome Engineering
• 刊名：Metabolic Engineering
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：39
• 期：Complete
• 页码：141-150
• 全文大小：1574 K
• 卷排序：39

文摘

Adaptive laboratory evolution enhanced tolerance towards L-serine from 3 to 100 g/L. Potential site of L-serine inhibition identified on homoserine dehydrogenase, ThrA. Gene clusters encoding potential serine exporters duplicated in evolved strains. Combined MAGE and amplicon sequencing enables identification of causative mutations. Evolved strain produced 37 g/L of L-serine with a 24% mass yield from glucose.