Structural analysis of the α subunit of Na⁺/K⁺ ATPase genes in invertebrates

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• 作者：Rahma Thabet^a ; J.-D. Rouault^b ; Habib Ayadi^a ; Vincent Leignel^c ; ^{Vincent.leignel@univ-lemans.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Alpha subunit ; Animal kingdom ; Evolution ; Genome ; Sodium&ndash ; potassium ATPase
• 刊名：Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology
• 出版年：2016
• 出版时间：June-July 2016
• 年：2016
• 卷：196-197
• 期：Complete
• 页码：11-18
• 全文大小：2287 K

文摘

The Na⁺/K⁺ ATPase is a ubiquitous pump coordinating the transport of Na⁺ and K⁺ across the membrane of cells and its role is fundamental to cellular functions. It is heteromer in eukaryotes including two or three subunits (α, β and γ which is specific to the vertebrates). The catalytic functions of the enzyme have been attributed to the α subunit. Several complete α protein sequences are available, but only few gene structures were characterized. We identified the genomic sequences coding the α-subunit of the Na⁺/K⁺ ATPase, from the whole-genome shotgun contigs (WGS), NCBI Genomes (chromosome), Genomic Survey Sequences (GSS) and High Throughput Genomic Sequences (HTGS) databases across distinct phyla. One copy of the α subunit gene was found in Annelida, Arthropoda, Cnidaria, Echinodermata, Hemichordata, Mollusca, Placozoa, Porifera, Platyhelminthes, Urochordata, but the nematodes seem to possess 2 to 4 copies. The number of introns varied from 0 (Platyhelminthes) to 26 (Porifera); and their localization and length are also highly variable. Molecular phylogenies (Maximum Likelihood and Maximum Parsimony methods) showed some clusters constituted by (Chordata/(Echinodermata/Hemichordata)) or (Plathelminthes/(Annelida/Mollusca)) and a basal position for Porifera. These structural analyses increase our knowledge about the evolutionary events of the α subunit genes in the invertebrates.