A genetic screen for increasing metabolic flux in the isoprenoid pathway of Saccharomyces cerevisiae: Isolation of SPT15 mutants using the screen
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- 作者：M. Wadhwa ; A.K. Bachhawat ; anand@iisermohali.ac.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：T ; TEF promoter ; C ; CYC promoter ; GGPPS ; Geranylgeranyl diphosphate synthase ; PSY1 ; phytoene synthase ; CRTI ; phytoene dehydrogenase
- 刊名：Metabolic Engineering Communications
- 出版年：2016
- 出版时间：December 2016
- 年：2016
- 卷：3
- 期：Complete
- 页码：164-172
- 全文大小：1157 K
文摘
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R. toruloides carotenogenic enzymes yield high β-carotene levels in S. cerevisiae.
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Phytoene dehydrogenase mutant (RtCRTIA393T) isolated through directed evolution.
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Preventing phytoene buildup by modulating the precursor enzyme GGPP synthase.
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Genetic visual screen for increased flux in isoprenoid pathway validated by tHMG1.
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Isolation of novel SPT15 mutants that show increased β-carotene and α-Farnesene.