Synchrotron X-ray microscopy reveals early calcium and iron interaction with crocidolite fibers in the lung of exposed mice
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- 作者：Lorella Pascoloa ; Giuliano Zabucchib ; Alessandra Gianoncellic ; George Kourousiasc ; Elisa Trevisanb ; Ernesto Pascottod ; Claudia Casarsab ; Chris Ryane ; Monica Lucattellif ; Giuseppe Lungarellaf ; Eleonora Cavarraf ; Barbara Bartalesif ; Marina Zweyerg ; Francesca Cammisulig ; Mauro Melatoa ; Violetta Borellib ; borelliv@units.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Asbestos ; Animal model ; Iron ; Calcium ; Synchrotron X-ray microscopy ; X-ray fluorescence
- 刊名：Toxicology Letters
- 出版年：2016
- 出版时间：22 January 2016
- 年：2016
- 卷：241
- 期：Complete
- 页码：111-120
- 全文大小：3203 K
文摘
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In a murine model of asbestos exposure a relevant inflammatory and atypical hyperplastic reaction was induced.
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Inflamed lung tissue regions of mice exposed to crocidolite, 1 month after treatment, were analyzed at two different synchrotron facilities: a high and a low energy microscopy set-up.
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Where conventional histochemical staining procedures fail to reveal any significant calcium and iron deposition, μ-XRF analysis is able to detect significant deposits of both iron and calcium on the macrophage ingested fibers.
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Asbestos fibers can induce iron and calcium ions sequestration early on following exposure.
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This process is concomitant with the appearance of inflammatory and hyperplastic reactions.