Pitfalls and promises in FTIR spectromicroscopy analyses to monitor iron-mediated DNA damage in sperm
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- 作者：Lorella Pascoloa ; lorella.pascolo@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Diana E. Bedollab ; c ; dianabedollaelettra@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Lisa Vaccarib ; lisa.vaccari@elettra.eu" class="auth_mail" title="E-mail the corresponding author ; Irene Venturina ; ireneventurin@hotmail.it" class="auth_mail" title="E-mail the corresponding author ; Francesca Cammisulia ; d ; francesca.cammisuli@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Alessandra Gianoncellib ; alessandra.gianoncelli@elettra.eu" class="auth_mail" title="E-mail the corresponding author ; Elisa Mitrib ; elisa.mitri@elettra.eu" class="auth_mail" title="E-mail the corresponding author ; Elena Giolod ; elena.giolo@burlo.trieste.it" class="auth_mail" title="E-mail the corresponding author ; Stefania Luppid ; stefania.luppi@burlo.trieste.it" class="auth_mail" title="E-mail the corresponding author ; Monica Martinellid ; monica.martinelli@burlo.trieste.it" class="auth_mail" title="E-mail the corresponding author ; Marina Zweyera ; zweyer@units.it" class="auth_mail" title="E-mail the corresponding author ; Giuseppe Riccia ; d ; giuseppe.ricci@burlo.trieste.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：FTIR ; Fourier transform infraRed ; XRF ; X-ray fluorescence ; ART ; assisted reproduction technology
- 刊名：Reproductive Toxicology
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：61
- 期：Complete
- 页码：39-46
- 全文大小：1771 K
文摘
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Oxidative stress effects in sperm can be monitored by FTIR microspectroscopy.
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Measurements on hydrated samples have many advantages and reveals artifacts.
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PBS and Iron-related precipitates may interfere with FTIR studies on DNA damage.