Glucosylceramide transferase in Giardia preferentially catalyzes the synthesis of galactosylceramide during encystation

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• 作者：Leobarda Robles-Martinez^a ; ^b ; ¹ ; ² ; Tavis L. Mendez^a ; ^b ; ¹ ; ² ; Jennifer Apodaca^a ; ^b ; Siddhartha Das^a ; ^b ; ^{sdas@utep.edu}
• 关键词：BLAST ; basic local alignment search tool ; CGlcT ; ceramide glucosyltransferase ; CGalT ; ceramide galactosyltransferase ; ESV ; encystation-specific vesicles ; gGlcT1 ; giardial glucosylceramide transferase 1 ; GlcCer ; glucosylceramide GalCer galactosylceramide ; GDP ; guanosine diphosphate ; GSL ; glycosphingolipid ; HMM ; hidden Markov model ; h.p.i.e ; hours post-induction of encystation ; KD ; knockdown ; NBD-ceramide ; N-[7-(4-nitrobenzo-2-oxa-1 3-diazole)]-ceramide ; PDI-2 ; protein-disulfide isomerase-2 ; TLC
• 刊名：Molecular and Biochemical Parasitology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：211
• 期：Complete
• 页码：75-83
• 全文大小：1901 K
• 卷排序：211

文摘

Glucosylceramide transferase (GlcT1) catalyzes the transfer of UDP-glucose to ceramide. The activity of giardial GlcT1 (gGlcT1) is upregulated during encystation. gGlcT1 is a dual substrate enzyme and utilizes both UDP-glucose and UDP-galactose. The rate of galactosylceramide synthesis by gGlcT1 is higher than glucosylceramide. gGlcT1 expression and cyst wall protein synthesis are co-regulated.