Increase in apoptotic effect of Panax ginseng by microwave processing in human prostate cancer cells: in vitro and in vivo studies

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• 作者：Jun Yeon Park¹ ; ^☆ ; Pilju Choi² ; ^☆ ; Ho-kyong Kim³ ; Ki Sung Kang¹ ; ^{kkang@gachon.ac.kr" class="auth_mail" title="E-mail the corresponding author} ; Jungyeob Ham² ; ^{ham0606@kist.re.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：anticancer ; ginsenoside ; microwave ; Panax ginseng ; prostate cancer
• 刊名：Journal of Ginseng Research
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：40
• 期：1
• 页码：62-67
• 全文大小：807 K

文摘

Ginseng, which is widely used in functional foods and as an herbal medicine, has been reported to reduce the proliferation of prostate cancer cells by mechanisms that are not yet fully understood.

Methods

This study was designed to investigate the changes in ginsenoside content in ginseng after treatment with a microwave-irradiation thermal process and to verify the anticancer effects of the extracts. To confirm the anticancer effect of microwave-irradiated processed ginseng (MG), it was tested in three human prostate cancer cell lines (DU145, LNCaP, and PC-3 cells). Involvements of apoptosis and autophagy were assessed using Western blotting.

Results

After microwave treatment, the content of ginsenosides Rg1, Re, Rb1, Rc, Rb2, and Rd in the extracts decreased, whereas the content of ginsenosides 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5 increased. Antiproliferation results for the human cancer cell lines treated with ginseng extracts indicate that PC-3 cells treated with MG showed the highest activity with an half maximal inhibitory concentration of 48 μg/mL. We also showed that MG suppresses the growth of human prostate cancer cell xenografts in athymic nude mice as an in vivo model. This growth suppression by MG is associated with the inductions of cell death and autophagy.

Conclusion

Therefore, heat processing by microwave irradiation is a useful method to enhance the anticancer effect of ginseng by increasing the content of ginsenosides Rg3, Rg5, and Rk1.