Continuous lactose fermentation by Clostridium acetobutylicum¡ªAssessment of energetics and product yields of the acidogenesis

详细信息    查看全文

• 作者：Fabio Napoli ; Giuseppe Olivieri ; Maria Elena Russo ; Antonio Marzocchella ; ^{antonio.marzocchella@unina.it} ; Piero Salatino
• 关键词：ABE ; Clostridium acetobutylicum ; Continuous culture ; ATP ; Yield ; Growth model
• 刊名：Enzyme and Microbial Technology
• 出版年：2012
• 出版时间：10 March 2012
• 年：2012
• 卷：50
• 期：3
• 页码：165-172
• 全文大小：576 K

文摘

An assessment of both the growth and the metabolism of acidogenic cells Clostridium acetobutylicum DSM 792 is reported in the paper. Tests were carried out in a CSTR under controlled pH conditions. Cultures were carried out using a semi-synthetic medium supplemented with lactose as carbon source. Acids and solvents, that represent products of the ABE process, have been purposely added in controlled amounts to the culture medium to investigate their effects on the product yields. The mass fractional yield of biomass and products were expressed as a function of the specific growth rate taking into account the Pirt model. The maximum ATP yield and the maintenance resulted 29.1 g_DM/mol_ATP and 0.012 mol_ATP/g_DM h, respectively. Quantitative features of the C. acetobutylicum growth model were in good agreement with experimental results. The model proposes as a tool to estimate the mass fractional yield even for fermentations carried out under conditions typical of the solventogenesis.